Elnaz Milani; Hashem Pourazerang; Shahrzad Vatankhah; Haneh Vakilian
Abstract
Inulin is widely used in functional foods throughout the world for its health-promoting and technological properties. Helianthus tuberosus is cultivated widely in the northern part of Iran. Helianthus tuberosus can be a valuable source of Inulin. To optimize conventional extraction of Inulin, various ...
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Inulin is widely used in functional foods throughout the world for its health-promoting and technological properties. Helianthus tuberosus is cultivated widely in the northern part of Iran. Helianthus tuberosus can be a valuable source of Inulin. To optimize conventional extraction of Inulin, various combinations of time, temperature, and solvent: solid ratio, were used. In this research, a series of statistically designed studies were performed to investigate the effect of each of the independent variables (temperature and pH of extraction medium, extraction time, and solvent: solid ratio). The following variables: solvent: solid ratio (5-12 v/w), temperature (40-90°C) and time (5-40 min) were studied. The central composite design (CCD) and response surface methodology (RSM) were employed as experimental design and statistical analysis. Based on canonical analysis, the optimal conditions for maximizing Inulin extraction yield (42.2%) were 76.64°C for 23.90 min and solvent: solid ratios of 12:1 (v/w).
Keywords: Inulin, Extraction, Helianthus tuberosus, Response surface methodology
Elnaz Milani; Hashem Pourazerang; Rassoul Kadkhodaee; Haneh Vakilian; Shahrzad Vatankhah
Abstract
Inulin is widely distributed throughout the plant kingdom. It has been increasingly used in various foods due to its beneficial nutritional attributes as prebiotic ingredient. The Helianthus tuberosus could be a source of inulin, but scarce studies about its extraction exist. Ultrasound has attracted ...
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Inulin is widely distributed throughout the plant kingdom. It has been increasingly used in various foods due to its beneficial nutritional attributes as prebiotic ingredient. The Helianthus tuberosus could be a source of inulin, but scarce studies about its extraction exist. Ultrasound has attracted considerable interest in food science and technology due to its promising effects in food processing and preservation. The use of power ultrasound significantly improves the extraction of organic compounds contained within the body of plants and seeds. The mechanical effects of ultrasound provide a greater penetration of solvent into cellular materials and improve mass transfer. In some cases sonication increased the efficiency of extraction at lower temperatures producing a purer product in a shorter time. It seems that the application of ultrasound would be a promising method to enhance the extraction process. The objective of this study was to develop a set of optimum extraction conditions for Helianthus tuberosus with an aim towards improving inulin extraction yield. Experiment design employed central composite design (CCD) and response surface methodology (RSM). Determination of inulin content was measured with the difference between total carbohydrate and reducing sugars. Then, Inulin extraction yield (%) was calculated too. Based on canonical analysis, the optimal conditions for maximizing inulin extraction yield (91.26%) were at 45.95 °C for 31.44 min and amplitude of 100%.
Keywords: Inulin, Extraction, Helianthus Tuberosus, Ultrasonic technique
